ABSTRACT
Objective:To evaluate the clinical significance of molecular detection testing multiple pathogens in children with viral central nervous system infections.Methods:We retrospectively included 176 children who were suspected with central nervous system infection at Shanghai Children′s Medical Center from January 2017 to May 2021.Film Array Meningitis/Encephalitis Panel(FA-M/E) was used to test cerebrospinal fluid samples of these children.The results were analyzed compared with clinical symptoms and cerebrospinal fluid indices.Results:There were 34 samples with positive FA-M/E virus detection(19.32%, 34/176). Among the 34 samples, enterovirus was the most common pathogen(27 cases, 79.41%). In different combinations, the sensitivity and positive predictive value were all less than 90%.The median time for antiviral drugs used in FA-M/E virus-positive and negative children was 4.5(0, 8.5)d and 2.6(0, 2.0)d, respectively.The difference was statistically significant( P<0.05). Conclusion:Molecular tests of multiple pathogens can quickly and sensitively detect pathogens.It can improve the efficacy of clinical diagnosis of viral central nervous system infection.
ABSTRACT
Objective@#To investigate the interaction between the gut microbiota and the sepsis in children by comparing the difference of gut microbiota between septic children and healthy children.@*Methods@#Genome was extracted from excrements of 18 cases of sepsis and 6 cases of healthy children.After genomic extraction, the hypervariable region of 16S rDNA gene were amplified and a small fragment library was constructed, and high-throughput sequencing was carried out, then the data of the lower machine was effectively sequenced by biological information processing.We could seek for the species that had changed significantly due to sepsis by comparing the diversity and the differences in the composition of intestinal flora between the two groups.@*Results@#The gut microbiome of the sepsis group was distinct from that of the health group.The operational taxonomic units in the sepsis group were significantly reduced compared with healthy group(P=0.001). The gut microbiome of children with sepsis had significantly lower diversity and richness compared with healthy group(P<0.05). A total of 7 species were shown to be differentially abundant between septic patients and healthy controls.The genus Pseudomonadales, Carnobacteriaceae and Granulicatella_elegans were significantly more abundant in the sepsis group; meanwhile the genus Pasteurellaceae, Ruminococcus, Lactobacillus_rogosae and Anaerostipes_butyratucus were less abundant in the sepsis group.In addition, the Granulicatella_elegans was characteristically present in the intestine of children with sepsis(P<0.001).@*Conclusion@#The microbial diversity and structure of the gut microbiome in children with sepsis are significantly different from those of healthy children.Our data suggest biomarkers identified in this study might participate in the pathogenesis or development process of sepsis.
ABSTRACT
Acute respiratory infection is a common infectious diseases in children.Rapid and accurate identification of pathogens is critical for targeted treatment and prognosis.Therefore, taking sensitive and effective detection methods is the key to pathogenic diagnosis.Now, the progress in the application and research of detection for respiratory pathogens in children were reviewed.
ABSTRACT
Objective To investigate the interaction between the gut microbiota and the sepsis in children by comparing the difference of gut microbiota between septic children and healthy children. Methods Genome was extracted from excrements of 18 cases of sepsis and 6 cases of healthy children. After genom-ic extraction,the hypervariable region of 16S rDNA gene were amplified and a small fragment library was constructed,and high-throughput sequencing was carried out,then the data of the lower machine was effec-tively sequenced by biological information processing. We could seek for the species that had changed signifi-cantly due to sepsis by comparing the diversity and the differences in the composition of intestinal flora between the two groups. Results The gut microbiome of the sepsis group was distinct from that of the health group. The operational taxonomic units in the sepsis group were significantly reduced compared with healthy group(P＝0. 001). The gut microbiome of children with sepsis had significantly lower diversity and richness compared with healthy group(P<0. 05). A total of 7 species were shown to be differentially abundant be-tween septic patients and healthy controls. The genus Pseudomonadales,Carnobacteriaceae and Granulicatella elegans were significantly more abundant in the sepsis group; meanwhile the genus Pasteurellaceae, Ruminococcus,Lactobacillus rogosae and Anaerostipes butyratucus were less abundant in the sepsis group. In addition,the Granulicatella elegans was characteristically present in the intestine of children with sepsis(P<0. 001). Conclusion The microbial diversity and structure of the gut microbiome in children with sepsis are significantly different from those of healthy children. Our data suggest biomarkers identified in this study might participate in the pathogenesis or development process of sepsis.
ABSTRACT
Acute respiratory infection is a common infectious diseases in children.Rapid and accurate identification of pathogens is critical for targeted treatment and prognosis.Therefore,taking sensitive and effective detection methods is the key to pathogenic diagnosis.Now,the progress in the application and research of detection for respiratory pathogens in children were reviewed.